The number of immune cells in and around kidney tumors, the amount of dead cancer tissue, and mutations to a tumor suppressor gene called PBRM1 form a biomarker signature that can predict — before treatment begins — how well patients with kidney cancer will respond to immunotherapy, according to new research directed by investigators at the Johns Hopkins Kimmel Cancer Center and its Bloomberg~Kimmel Institute for Cancer Immunotherapy.
In reviews of 136 kidney tumor biopsies taken for previous studies, investigators found that patients who had three positive factors — presence of immune cells in and around tumors, known as tumor-infiltrating immune cells, absence of dead cancer tissue, called necrosis, and a mutation in the PBRM1 gene — had the best overall survival at five years compared with patients who did not have this combination of factors. A report about the work was published online Feb. 21 in the journal Cell Reports Medicine.
Therapies for patients with metastatic clear cell renal carcinoma, a type of kidney cancer, are rapidly evolving and include immunotherapy-based regimens, says lead study author Julie Stein Deutsch, M.D., a clinical fellow in dermatopathology at the Johns Hopkins University School of Medicine. However, there is an unmet need for biomarkers that can help match patients to the regimens most likely to help, she says. Such markers have been investigated in lung and other cancers, but have not shown the same predictive ability in patients with kidney cancers.
The classic hematoxylin and eosin-stained (H&E) pathology slide — the gold standard for diagnosing and staging cancer in medical practices worldwide — has largely been overlooked as a possible source of biomarker information, Deutsch says. “There are many studies investigating biomarkers for response to immunotherapy using advanced technologies that require expensive machines and experienced technicians. The ability to use information from an H&E slide, pretreatment, to predict overall survival of patients receiving this therapy is extremely powerful, and is something that can be used in resource-poor settings as well,” she says.
Immunotherapies that target PD-1 (programmed cell death 1), a protein found on immune cells, can unleash an immune response against cancer cells and has become a vanguard of cancer therapy, says senior author Janis Taube, M.D., M.Sc., co-director of the Tumor Microenvironment Laboratory at the Bloomberg~Kimmel Institute for Cancer Immunotherapy and director of the Division of Dermatopathology. However, she points out that anti-PD-1 immunotherapies do not work in all patients. The new findings could be used to help preselect patients for the most appropriate therapy, says Taube.
Investigators examined H&E slides from 136 metastatic tumor samples, before treatment, from patients with renal cell cancers to determine the biomarker potential of this commonly available material. They reviewed 63 biopsies obtained before treatment from patients who received the immunotherapy nivolumab as a first cancer treatment or later treatment; 58 biopsies from patients receiving later-line nivolumab or the chemotherapy drug everolimus; and 15 biopsies from patients who hadn’t received therapy before, who received nivolumab plus the immunotherapy ipilimumab. Researchers scored the specimens for the amount of tumor-infiltrating immune cells (here called TILplus) and presence of necrosis (dead tissue).
In the first group of 63 biopsies, and in samples from all three groups of patients who received immunotherapy, patients with specimens that had immune infiltrates (e.g., tumor-infiltrating lymphocytes, macrophages, plasma cells) interfacing with tumor (TILplus score of 1) showed improved overall survival compared with those without (i.e., specimens with TILplus score of 0). Median overall survival was 47.9 months in those with a TILplus score of 1 versus 16 months in those with a score of 0. Median progression-free survival was 7.5 months in those with a TILplus score of 1 versus 2.7 months in those with a score of 0. However, TILplus score was not associated with overall survival among patients receiving everolimus, indicating the findings were specific to immunotherapy.
The presence of necrosis was found to modify the benefits of having immune system infiltration in the tumor. In two groups of biopsies studied, patients whose tumors had substantial necrosis (greater than 10% surface area) had lower overall survival compared with patients who had the same TILplus score but whose tumors lacked necrosis. This finding was observed in patients from two cohorts. Again, combining TILplus and necrosis scores was not predictive of outcomes for patients receiving everolimus.
“This is important, because traditionally, areas of necrosis are often excluded from biomarker studies because it can’t be used for genomic or transcriptomic studies since the tissue is dead,” Deutsch says. “We show there is important information in that necrotic area that’s conferring some sort of negative disadvantage to patients. It’s important not to overlook these areas when you’re investigating biomarkers that predict how patients are going to do.”
Finally, investigators looked at mutations in the PBRM1 gene and how that impacts the other factors. Such mutations were correlated with overall survival but were not associated with TILplus. However, a statistical analysis of all three factors found that the combination of H&E scoring with PBRM1 mutation status stratified patients into three groups. Patients who had all three positive factors — a TILplus score of 1, necrosis score of 0 and a PBRM1 mutation — had the best overall survival at five years. Patients with two of the three features demonstrated intermediate survival, while those with only one feature had the worst survival.
When investigators performed a literature search, they were unable to find other studies that used H&E features as part of tumor characterization using multimodality approaches. “This demonstrates the underutilization of these insights in biomarker discovery for immunotherapies,” Deutsch says. (PB/Newswise)