![The study reveals that the opportunistic human fungal pathogen Candida albicans requires the ability to metabolize proline, an amino acid obtained from the host, to mount virulent infections(Representational image: Unsplash)](http://media.assettype.com/medbound%2F2023-11%2Fa4af815d-6746-4d6f-bc0d-bb6afe80c6bc%2Fiiii.jpg?w=480&auto=format%2Ccompress&fit=max)
An international team of scientists led by researchers from the Department of Molecular Biosciences, The Wenner-Gren Institute, SciLifeLab, Stockholm University has published in PLoS Pathogens the first successful application of 2-photon intravital microscopy (IVM) to image the dynamics of fungal infections in the kidney of a living host. The study reveals that the opportunistic human fungal pathogen Candida albicans requires the ability to metabolize proline, an amino acid obtained from the host, to mount virulent infections.
Candida albicans has recently been listed as one of four “critical priority” fungal pathogens by the World Health Organization (WHO). A defining feature of C. albicans is that it is a commensal organism that thrives in symbiosis along with other components of the human microflora, and is normally well-tolerated.
However, when humans experience health challenges that negatively affect the immune system, C. albicans can cause bloodstream infections that are lethal unless aggressively treated. During infection, C. albicans cells are known to switch morphologies from ovoid yeast-like to elongated filamentous hyphal cells, a process linked to the capacity of this fungi to grow as a pathogen. Although proline has long been known to trigger hyphal growth, we have recently discovered that C. albicans can use proline as a main source of energy. Proline breakdown takes place in the mitochondria, the powerhouse of the cell.
The investigating team now report that proline is an important source of energy in other pathogenic Candida species, including the multidrug resistant C. auris, an emerging health threat and also a WHO critical priority fungal pathogen.
The collaborating groups provided access to a multitude of infection models, including artificial skin, co-culture with immune cells, survival in whole human blood and two model host systems. The results consistently showed that strains unable to metabolize proline exhibit significantly reduced virulence properties and a clearly diminished capacity to undergo morphological transitions. These observations provide novel insights implicating proline metabolism as a key determinant of pathogenic fungal growth. Proline, one of the 20 naturally occurring amino acids in the body, is enriched in extracellular matrix proteins, e.g., collagen, and as such is readily available when connective tissue is broken down at sites of infection, or when a host becomes vulnerable as a result of cancerous growth or upon the onset of sarcopenia. Strikingly, genetic dissection of the Proline UTilization (PUT) pathway and of the control mechanisms governing proline use led to the discovery that proline is toxic to cells unable to catabolize it. This latter finding was unexpected and further work is needed to solve the mystery and underlying mechanism of its toxicity.
The kidney is the primary organ affected during bloodstream infections by C. albicans, and it is imperative to understand why. To obtain answers, the team initially applied a mouse model of infection, which remains invaluable for this kind of work, and found that C. albicans cells lacking the capacity to utilize proline were less invasive and virulent. Notably, mice infected with fungal cells unable to catabolize proline, e.g., cells lacking the Put2 enzyme (1-pyrroline-5-carboxylate (P5C) dehydrogenase), showed milder illness or no symptoms at all. Next, state-of-the-art 2-photon intravital microscopy (IVM) was used to visualize, in real-time, the invasion of C. albicans cells deep in the cortex of the kidneys inside a living host. In contrast to native wild-type cells, the Put2-deficient cells (put2-/-) failed to form hyphae in kidneys. It should be recognized that imaging the initial stages of an ongoing infection is very challenging due to many factors, such as the small size and scarcity of the fungal cells and the continuous motions associated with vital and life-sustaining processes in living hosts.
(VP/Newswise)